Composite

Part:BBa_K136019:Design

Designed by: Kok-Phen YAN   Group: iGEM08_Paris   (2008-10-25)


weak promoter - GFP tripart


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 708


Design Notes

gfp tripart was digested by XhoI and PstI (backward insert) and cloned into the plasmid J61002 containing the weak promotor.


Source

Assembly of different parts from the registry.

References